Figure 6

N-acetyl-L-cysteine (NAC) suppressed programmed cell death and inflammation promoted by Nox4 overexpression in cisplatin (Cis)-treated cells. (a, b) Western blotting and real-time PCR of KIM-1. NAC suppressed the level of KIM-1 protein promoted by Nox4 overexpression in Cis-treated cells, which was further confirmed at the mRNA level. (c) Real-time PCR of inflammatory indices. NAC suppressed Nox4-upregulated mRNA level of TNF-α, IL-1β and IL-6 in Cis-treated HK2 cells. (d) Western blotting of programmed cell death-related molecules. NAC suppressed Nox4-upregulated RIPK1, RIPK3, P-MLKL and cleaved-caspase3 in Cis-treated cells. Data represent the mean±s.e.m. for 3–4 independent experiments in vitro. *P<0.05, **P<0.01, ***P<0.001 versus normal; ^#P<0.05, ^##P<0.01, ^###P<0.001 versus Nox4-EV group; ^$P<0.05, ^$$P<0.01, ^$$$P<0.001 versus cisplatin-treated group.