Figure 7
Apocynin (Apo) prevented cisplatin (Cis)-induced renal injury, supported renal function and decreased KIM-1 and reactive oxygen species (ROS) in vivo. (a) Malondialdehyde (MDA) assay in mice. Apo significantly reduced the level of ROS in Cis nephropathy. (b) Western blotting analysis of Nox4. Intraperitoneal injection of Apo significantly reduced the Cis-upregulated Nox4 protein level. (c) Periodic acid-Schiff (PAS) staining. Apo prevented Cis-induced renal injury in vivo. (d, e) Renal function. Results of serum creatinine and BUN showed that Apo suppressed the decline of renal function caused by Cis. (f) Real-time PCR of KIM-1. Apo reduced the mRNA level of KIM-1 in Cis nephropathy. (g and h) Western blotting analysis and immunohistochemistry (IHC) of KIM-1. Apo reduced the protein level of KIM-1 protein in Cis nephropathy, which was further confirmed by IHC. Data represent the mean±s.e.m. for 6–8 independent experiments in vivo. *P<0.05, ***P<0.001 versus normal; #P<0.05, ##P<0.01, ###P<0.001 versus cisplatin-treated group. Noxs: NADPH oxidases.
