Figure 9
Lentivirus-mediated knockdown of Nox4 protected against renal injury in cisplatin nephropathy. (a) Immunohistochemistry and quantitative analysis of Nox4 in mice. (b) Real-time PCR of Nox4 in mice. Nox4 protein was increased in cisplatin nephropathy, which was further confirmed by real-time PCR for mRNA. (c) Western blotting and quantitative analysis of Nox4 in mice. Nox4 was significantly upregulated in a time-dependent manner in response to cisplatin. (d) Nox4 knockdown in mice. Western blotting analysis showed a substantial knockdown of Nox4 protein in the kidney. (e) PAS staining. Silencing Nox4 attenuated cisplatin-induced kidney damage in vivo. (f, g) Detection of renal function. Results of serum creatinine and BUN show that knockdown of Nox4 protected against the loss of renal function in cisplatin nephropathy. Data represent the mean±s.e.m. for 6–8 independent experiments in vivo. *P<0.05, **P<0.01, ***P<0.001 versus normal. ##P<0.01 versus vector control mice.
