Figure 1
From: Suppression of islet homeostasis protein thwarts diabetes mellitus progression
Islet homeostasis protein (IHoP) expression in mouse and human pancreatic islets. (a) IHoP immunoprecipitation with protein extracted from αTC1.9 cells (mouse-derived alpha cell line); arrow designates a single band product at 63 kDa. (b) Normal mouse (left panels) and diabetic NOD mouse islets (right panels; post-onset) stained for glucagon (brown) and IHoP (brown). (c) Normal human and type 1 diabetes patient pancreas tissues stained for glucagon (brown) and IHoP (brown) (Pancreas tissue was a generous gift from Dr Roberto Gianami, University of Colorado Health Science Center, Denver, CO, USA). Nuclei counterstained with hematoxylin (blue). Scale bars, 50 μm (b) and 20 μm (c). (d) Co-localization of glucagon and IHoP expression in pancreatic tissue from a post-onset NOD mouse and a type 1 diabetes patient. Post-onset NOD mouse and type 1 diabetes patient pancreas tissues stained for glucagon (red), IHoP (green), and merged (yellow). Nuclei were counterstained with DAPI (blue). Scale bars, 20 μm.
