Figure 1 | Laboratory Investigation

Figure 1

From: Drugs with anti-oxidant properties can interfere with cell viability measurements by assays that rely on the reducing property of viable cells

Figure 1

(a) Cell viability assay of the RCC4 cell line treated with Azacytidine and the drug of interest (DOI) with known anti-oxidant properties with the Cell Titer Blue protocol. As per manufacturer’s instructions the Cell Titer Blue reagent was added directly to the media with the treatment drugs. The results suggest that the DOI may be enhancing viability (two-tailed t-test ‘P’ values for DOI 5 mM and DOI 5 mM+aza in comparison to control—7.7E−06 and 1.6E−05, respectively). (b) Same protocol and cell line (RCC4) except removal of the media containing the drug and washing the cells prior to incubation with fresh media and Cell Titer Blue reagent. This reveals the drug to be reducing cell viability (‘P’ values for DOI 5 mM and DOI 5 mM+aza in comparison to control—0.1 and 0.004, respectively). (c, d) Same experiment as (a and b) on a different RCC cell line 786-O. (c) ‘P’ values for DOI 5 mM and DOI 5 mM+aza in comparison to control—0.0004 and 0.009, respectively. (d) ‘P’ values for DOI 5 mM and DOI 5 mM+aza in comparison to control—0.04 and 0.01, respectively.

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