Figure 2 | Laboratory Investigation

Figure 2

From: Drugs with anti-oxidant properties can interfere with cell viability measurements by assays that rely on the reducing property of viable cells

Figure 2

Representative picture of the Cell Titer Blue assay with a lymphoma cell line LY-1. The blue wells (nonfluorescent) are control or Azacytidine treatment wells, the purple wells (medium fluorescence) are with 1 mM drug of interest, and the pink wells (highest fluorescence) are with 5 mM drug of interest, without and with Azacytidine; falsely implying that the viability of the cells increased with increase in drug concentration. The bar graph represents the wells in the picture (two-tailed t-test ‘P’ values for DOI 1 mM, DOI 5 mM, and DOI 5 mM+aza in comparison to control—1.16E−08, 4.7E–14, and 2.27E−13, respectively).

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