Figure 1
From: APX-115, a first-in-class pan-NADPH oxidase (Nox) inhibitor, protects db/db mice from renal injury
Effect of APX-115 on oxidative markers. (a) Plasma 8-isoprostane level. (b) Urine 8-isoprostane level. (c) Kidney tissue lipid peroxidase (LPO) level. (d) Fat tissue LPO level. (e) Liver tissue LPO level among groups. (f) Kidney tissues from untreated or APX-115 group of db/m or db/db mice were embedded and freshly cut at 10 μm thickness. Tissue slides were stained with dihydroethidium (DHE, 5 μ M, Molecular Probes) for 10 min at 37 °C to evaluate superoxide production with red fluorescence (585 nm) by Carl Zeiss vision system (LSM510 meta). (g) Quantification of DHE level in (f). *P<0.05, **P<0.01, ***P<0.001 vs db/m groups. ##P<0.01, ###P<0.001 vs db/db control.
