Figure 6

Effect of knocking out Nox isoforms on TGF-β-induced ROS generation in primary cultured mesangial cells. Primary mouse mesangial cells (pMMCs) from wild-type (WT), Nox1 knockout (KO), Nox2 KO, Nox4 KO, Duox1 KO, and Duox2 KO mice were serum starved for 6 h, stimulated with TGF-β1 (10 ng/ml) for 10 min, and then incubated within 2', 7'-dichlorofluorescindiacetate (DCF-DA) for 10 min. ROS generation from each sample was monitored by confocal microscopy (N=3, data shown as mean±s.d.).