Figure 5 | Laboratory Investigation

Figure 5

From: Increased expression of latent TGF-β-binding protein 4 affects the fibrotic process in scleroderma by TGF-β/SMAD signaling

Figure 5

Transfection of LTBP-4 siRNA into scleroderma skin fibroblasts induces decreased collagen expression. (a) RT-PCR showed LTBP-4, COL1A1, COL1A2, and other fibrosis-related gene expression 48 h after knockdown treatment with LTBP-4 siRNA, and the mRNA levels were calculated using a relative ratio to GAPDH. (b) LTBP-4 and collagen type I protein expression levels were assessed by western blot and GAPDH was used as a reference control for equal protein loading. (c) Densitometry data of the protein were analyzed in two groups. Bars represented mean±s.e.m. in each group. ***P<0.0001, **P<0.01, *P<0.05. LTBP-4, latent TGF-β-binding protein 4; RT-PCR, real-time PCR.

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