Figure 2

Differential glycan profiling of sialylated MUC1 and multivariable analysis between 21 cholangiocarcinoma (CCA) and 50 pancreatic ductal adenocarcinoma (PDAC) patients. (a) Glycan profiles of 21 CCA and 50 PDAC patients listed in Table 1 with 45 lectins and a typical image of immunostaining with MY.1E12. The horizontal line inside each box represents the median, and the lower and upper borders of the box encompass the interquartile range. Vertical lines from the ends of each box encompass the 5th and 95th percentiles. (b) Hierarchical clustering analysis using the glycan profiling data of all CCA and PDAC formalin-fixed paraffin-embedded tissue samples. The heat map shows a two-way cluster analysis carried out on the data of the tissue specimens listed in Table 1. The data were obtained as averages of multiple tissue samples. Levels of lectin signals are indicated by color changes from blue (high expression level) to red (low expression level). CCA and PDAC patients are indicated by blue and red lines, respectively. (c) Principal component analysis of glycan profiling of MUC1 in 21 CCA and 50 PDAC patients. CCA and PDAC are indicated in red and blue, respectively. (d) Relative intensity of 45 lectins shown as a biplot and bar graphs.