Figure 4
From: Assessment of tumor characteristics based on glycoform analysis of membrane-tethered MUC1
Lectin-MY.1E12 double fluorescent immunostaining of cholangiocarcinoma (CCA) and pancreatic ductal adenocarcinoma (PDAC) formalin-fixed paraffin-embedded sections. Double fluorescent immunostaining with two lectins (MAL and PWM) shows significant differences between CCA and PDAC and MY.1E12 and between WFA and MY.1E12 as a control. Each lectin was labeled with Alexa 592 streptavidin (red), and MY.1E12 was labeled with Alexa 488 anti-mouse IgG (green). Marginal areas are shown in yellow. Nuclei are visualized with Hoechst 33258 (blue). Each bar indicates 20 or 200 μm. HE staining of tissue specimens using double fluorescent immunostaining is shown in Supplementary Figure S4.
