Figure 2

In vivo imaging evaluation of peritoneal permeability in mice. A selected group of mice (dialysis group) were treated with PDF during 30 days. At the end of the experiment, FITC-dextran was instilled into the mouse peritoneal cavity and body fluorescence images were acquired every 30 s (a). The mean pixel intensities of the ROIs of the nose (above) and paws (below) were determined and demonstrated that the fluorescence intensity increased over time in both groups. However, the mean pixel intensity increased faster in the dialysis group compared to the control group (b). The in vivo calibration curve was used to determine the circulating FITC-dextran (c). The result shown is representative of two experiments (n=4 mice per group).