Figure 3 | Laboratory Investigation

Figure 3

From: Whole tumor section quantitative image analysis maximizes between-pathologists’ reproducibility for clinical immunohistochemistry-based biomarkers

Figure 3

Whole tumor section approach. Morphologic differences between field-of-view and whole tumor section image analysis annotation approaches (top panel). Fields-of-view are drawn to scale. Whole tumor section captures relevant scoring areas, in this case, all invasive tumor cells. Overall percent agreement vs absolute difference between average positive and negative between-reader agreements for whole tumor section annotated cases (bottom panel). For each biomarker, the three dots represent the three reader (R) pairs (R1 vs R2, R1 vs R3, and R2 vs R3). For Ki-67 and HER2, all three dots for each marker are overlapping at 100%; for ER at 98.3%; for PR at 98.3% for two dots.

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