Figure 3 | Laboratory Investigation

Figure 3

From: Activation of pluripotent genes in hepatic progenitor cells in the transition of nonalcoholic steatohepatitis to pre-malignant lesions

Figure 3

Enhanced inflammation in the liver of mice fed HFC diet plus HF/G. (a–d) Upregulation of TNF-α and IL-1β at mRNA and protein levels in mice fed HFC diet plus HF/G over time. (e) CD163 immunofluorescent staining for activated Kupffer cells in the liver of mice fed HFC diet plus HF/G overtime. Images were taken at original magnification (× 400). Scale bars=50 μm. (f) Quantitative count of CD163-positive cells in the liver of mice fed HFC diet plus HF/G overtime. (g) α-SMA immunohistochemical staining for activated hepatic stellate cells (HSCs) in mice. Images were taken at original magnification (× 400). Scale bars=50 μm. (h) Semi-quantitative analysis of α-SMA-positive cells in the liver mice fed HFC diet plus HF/G overtime. *P<0.05, **P<0.01 compared to mice fed control diet at corresponding time points.

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