Figure 6 | Laboratory Investigation

Figure 6

From: Activation of pluripotent genes in hepatic progenitor cells in the transition of nonalcoholic steatohepatitis to pre-malignant lesions

Figure 6

Enhanced expression of pluripotent c-Myc, Oct-4, and KLF-4 genes in hyperplastic dysplasia tissue. C57BL/6J mice were fed a control diet or high-fat/-calorie diet plus high fructose/glucose in drinking water (HF/C diet plus HF/G) for 12 months. (a–c) Representative micrographs and semi-quantification (d–f) of immunohistochemical staining of c-Myc, Oct-4, and KLF-4 in mouse liver at 5, 9, and 12 months. The positive signal was developed with DAB (in brown color indicated by arrow symbols). Representative images were recorded at original magnification (× 400), and scale bars=50 μm. *P<0.05 and **P<0.01 compared to mice fed control diet at corresponding time point.

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