Figure 7 | Laboratory Investigation

Figure 7

From: Activation of pluripotent genes in hepatic progenitor cells in the transition of nonalcoholic steatohepatitis to pre-malignant lesions

Figure 7

Enhanced expression of pluripotent Nanog gene and morphogenic Gli-1 gene in hyperplastic dysplasia tissue. C57BL/6J mice were fed a control diet or high-fat/-calorie diet plus high fructose/glucose in drinking water (HF/C diet plus HF/G) for 12 months. (a, b) Representative micrographs and semi-quantitation (c, d) of immunohistochemical staining of Nanog and Gli-1 in mouse liver at 5, 9, and 12 months. The positive signal was developed with DAB (in brown color indicated by arrow symbols). Representative images were recorded at original magnification (× 400), and scale bars=50 μm. *P<0.05 and **P<0.01 compared to mice fed control diet at corresponding time point.

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