Figure 8

Co-localization of c-Myc, KLF-4, and Nanog in hepatic oval cells in hyperplastic dysplasia tissue. C57BL/6J mice were fed a control diet or high-fat/-calorie diet plus high fructose/glucose in drinking water (HF/C diet plus HF/G) for 12 months. (a) c-Myc, (b) KLF-4, (c) Nanog. Representative micrographs of fluorescent immunohistochemical co-staining of OV-6 (red) with c-Myc (green), KLF-4 (green), or Nanog (green) in mouse liver after feeding for 12 months. DAPI was used for nucleus staining (blue). Yellow color represents the overlapping of oval-6 with c-Myc, KLF-4, or Nanog. Images were recorded at original magnification (× 400). Scale bars=50 μm. HV, hepatic vein.