Table 1 List of IGH and IGK probes assessed in patient-specific MRD tests

From: Improving minimal residual disease detection in precursor B-ALL based on immunoglobulin-κ and heavy-chain gene rearrangements

Group

Target

Probe

Sequence (5′–3′)

Comment

IGH (V)

VH1

VH1co.MGB

AGCCTACATGGAGCTGA

 
 

VH1

VH1co.LNA

AG+CCT+AC+ATGG+AG+CTGA

 
 

VH2

VH2eb.MGB

ATGACCAACATGGACC

 
 

VH3

VH3fr.MGB

TCTGCAAATGAACAGCCTGA

 
 

VH3

VH3fr.LNA

TCTG+CAA+ATG+AA+CAG+CCTGA

 
 

VH4

VH4sh.MGB

TCTCCCTGAAGCTGAGCT

 
 

VH4

VH4sh.LNA

TCT+CC+CTGA+AGCTG+AGCT

 
 

VH6

VH6nsh.MGB

TCTCCCTGCAGCTGAACT

 

IGH (J)

J1,2,4,5

JH1245.ABI

CCCTGGTCACCGTCTCCTCAGGTG

Verhagen et al.3

 

J3

JH3.ABI

CAAGGGACAATGGTCACCGTCTCTTCA

Verhagen et al.3

 

J6

JH6.ABI

CACGGTCACCGTCTCCTCAGGTAAGAA

Verhagen et al.3

IGK (V)

VK1

VK1.MGB

AGCCTGCAGCCTGAA

 
 

VK1

VK1.LNA

AG+CC+TGCAG+CC+TGAA

 
 

VK2

VK2.ABI

ACACTGAAAATCAGCAGGGTGGAGGCT

 
 

VK3

VK3.ABI

AGCAGACTGGAGCCTGAAGATTTTGCAG

 

IGK (Kde)

Kde

Kde.ABI

AGCTGCATTTTTGCCATATCCACTATTTGGAG

van der Velden et al.2

IGK intron

Kintron

KRSS.ABI

GAGCCGCGGTCTTTCTCGATTGAGT

Not recommended

  1. Abbreviations: IGH, immunoglobulin heavy chain; IGK, immunoglobulin-κ; MRD, minimal residual disease.
  2. The probe names include one of the three suffixes to indicate their chemical structure. ABI is used for Taqman probes with TAMRA synthesized by Applied Biosystems, Foster City, California, USA; MGB is for probes with major groove-binding capacity made by ABI and LNA is for Locked Nucleotide probes made by Proligo, Paris, France. Data for the four pairs of LNA and MGB probes were equivalent and combined for analysis. The VH3 probe is based on longer sequence published by Kwan et al.,5 and the J and Kde probes have been published previously.2, 3
Back to article page