Figure 3
Cytokine-mediated apoptosis in ST4-S1 cells. (a) ST4-WT, ST4-C and ST4-S1 cells were treated with interferon (IFN)-γ (•), interleukin (IL)-6/sIL-6R (▾) or left untreated (▪) until 120 h. At 24, 48, 72 and 120 h, cell aliquots were recovered and stained with Annexin-V-phycoerythrin to detect apoptotic cells by FACS analysis. The percentages of Annexin-V-positive cells are shown as means ± s.e.m. from two independent experiments at each time point. (b) After 48 h of cytokine treatment, total proteins were extracted from cells and the presence of cleaved and uncleaved forms of caspase 3 was detected by western blot. Actin was used as a control for equal protein loading.
