Figure 2

mBAFF mediates the specific binding and internalization of PinX1C-containing fusion proteins into BAFF receptor-positive cells. The BAFF receptor-positive cells including Raji (a), Namalwa (b), Daudi (c), JeKo-1 (d) and THP-1 (e), and BAFF receptor-negative Jurkat cells (f) were treated with 250-nM fusion proteins for 1 h. The cells were then adhered onto a microscope slide and fixed in 4% paraformaldehyde, followed by a brief rinse with PBS. Subsequently, the cells were incubated with an anti-His monoclonal antibody. After a brief wash with PBS, cells were incubated with FITC-conjugated goat antimouse IgG. After washing with PBS, slides were mounted in mounting medium and then analyzed by laser scanning confocal microscopy.