Figure 1 | Leukemia

Figure 1

From: Janus kinase 2 regulates Bcr–Abl signaling in chronic myeloid leukemia

Figure 1

Knockdown of Jak2 strongly reduced levels of the Bcr–Abl protein and pTyr 177 of Bcr–Abl in Bcr–Abl+ cells and CML cell lines. (a) Knockdown of Jak2 and rescue of Jak2 expression in Bcr–Abl+ 32D cells (32Dp210) using Jak2-specific small interfering RNA. 32Dp210 contains a b3a2 form of Bcr–Abl expressed in mouse myeloid 32D cells. (b) Knockdown of Jak2 in K562-R CML cell line by an inducible Jak2-specific short hairpin RNA. Jak2 knockdown was initiated by addition of doxicycline (2 μg/ml) for 3 days. (c) Non-targeted short hairpin RNA has no effects on Jak2 expression and Bcr–Abl expression. (d) Reversal of Jak2 knockdown restores levels of pTyr177 and the Bcr–Abl protein. After a 3-day induction, doxicycline was withdrawn from the culture for 6 days. Western blotting with the appropriate antibodies was performed in these studies.

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