Figure 1 | Leukemia

Figure 1

From: A unique three-dimensional SCID-polymeric scaffold (SCID-synth-hu) model for in vivo expansion of human primary multiple myeloma cells

Figure 1

(A) SEM analysis of a PCLS ( × 65 (1), × 500 (2), × 650 (3) and × 1200 (4) magnification) shows interconnected large and small pores (white bars=1 mm (1), 200 μm (2), 100 μm (3) and 50 μm (4)). (B) Comparative SEM analysis ( × 150 magnification) between a synthetic PCLS (upper panel) and a surgical sample of human femur adult bone (lower panel) shows similar microarchitecture (white bars=500 μm). Surface morphology was studied by a Leica Cambridge (Stereoscan S440) SEM (Cambridge, UK) at an accelerating potential of 20 kV. (C) H&E staining of adherent OP9 mouse stromal cells at 3 weeks after scaffold implant in mice. (D–F) SEM analysis ( × 3500 magnification) (d), H&E staining (e) and confocal laser scanning microscopy (f) show adherence within a PCLS of human BMSCs, obtained from two different patients (pt #3, (a); pt #7, (b) See Table 1), 1 week after implant in mice. (G) Flow chart diagram. Schematic representation of experimental methodology.

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