Figure 5

Effect of LBH589 on proteins characteristic of adult T-cell leukemia/lymphoma (ATLL) cells. Cells were treated with either vehicle or 50 nM of LBH589 for 24 h. After cells were harvested, flow cytometric analysis (FCM), real time quantitative RT-PCR and western blotting were performed. (a and b) Changes in expression of CCR4 and IL-2R were evaluated by FCM. The results were indicated using RFI. (c–e) Real-time quantitative RT-PCR and western blotting against HTLV-1 Tax, HBZ and HBZ-SI were performed as described in materials and methods. RT-PCRs were carried out in duplicate and the average value was used as the absolute amount of each mRNA. The cells were divided into three groups according to the amount of Tax mRNA; cell lines with high Tax mRNA levels (KOB, MT2 and HuT102), moderate levels (LM-Y1) and low levels (KK1 ST1 and SO4). The results of western blotting are also shown in each panel. (b–e) Results were expressed as the mean±s.d. for three independent experiments and were also analyzed using Student's t-test. ^*P<0.01.