Figure 1
From: Syndecan-1 promotes the angiogenic phenotype of multiple myeloma endothelial cells
Characterization of HUVECs, BMECs and MMECs assessed by flow-cytometric, immunofluorescence and qRT-PCR analysis. (a) Representative flow-cytometric analysis showing isolated MMECs expressing CD31 and ulex europaeus agglutinin-1 (UEA-1) but not CD14 and CD38. The flow-cytometric histograms are representative of six independent experiments with similar results. The dark lines represent the MMECs and the dotted lines represent the corresponding isotype control antibody. (b) Representative confocal micrographs of vWF, vimentin and VE-cadherin expression in BMECs and MMECs detected by immunofluorescence. Original magnification: × 400. (c, d) Comparison of syndecan-1 mRNA expression by HUVECs, BMECs and MMECs using qRT-PCR (c) and flow cytometry (d). (c) The normalized expression of the genes with respect to ACTB was computed for all samples. Values are expressed as fold change with respect to HUVECs and are the mean±s.d. of six independent experiments performed in triplicate. Student's t-test was performed (*P<0.001 MMECs versus HUVECs; †P<0.05 MMECs versus BMECs). (d) Representative flow-cytometric analysis showing syndeacan-1 expression in HUVECs (dotted line), BMECs (thin line) and MMECs (dark line). The flow-cytometric histograms are representative of six independent experiments with similar results.
