Figure 4
From: Syndecan-1 promotes the angiogenic phenotype of multiple myeloma endothelial cells
Syndecan-1 expression is associated with invasion properties of MMECs. (a) Invasion of HUVECs, MMECs and MMEC syndecan-1 siRNA and, as control, MMEC scr-siRNA towards Matrigel-coated filters was evaluated. Cells that migrated to the underside of the filters were counted in five microscope fields in each well at × 100 magnification. Data are expressed as the mean±s.d. of four experiments performed in duplicate. Analysis of variance with Dunnett multiple comparison test was performed (*P<0.001 MMECs versus HUVECs; †P<0.001 MMEC syndecan-1 siRNA versus MMECs). (b) Representative zymographic analysis of MMECs and MMEC syndecan-1 siRNA. The first lane (FBS) shows the control RPMI supplemented with 10% FBS. The clear bands represented gelatinase activity. Four experiments were performed with similar results. (c) Evaluation of MMECs and MMEC syndecan-1 siRNA attachment on growth factor-reduced Matrigel after 2 h in media used for invasion assay (not complete EBM with 10% FBS). Data are expressed as ±s.d. of three different experiments each in triplicate. Student's t-test was performed (*P<0.001).
