Figure 1
From: Differential regulation of miR-21 and miR-146a by Epstein–Barr virus-encoded EBNA2
Global miR expression profile by microarray and qPCR of EBV-infected and EBNA2-transfected U2932: The experimental samples and the vector-transfected U2932 were labeled with Hy5 and Hy3, respectively. Three different RNA preparations were generated from the controls, EBV-infected and EBNA2-transfected cells. Red signals represent increase and the green color represents miRs whose expression is downregulated. Custom-made slides containing LNA-based capture probes (Exiqon library from the miR BASE version 8.1) representing >460 miRs were spotted. (a) Two EBV-infected clones of U2932 cells, U2932 EBVGFP cl-A (EBNA2 negative) and U2932 EBVGFP cl-B (EBNA2 positive), were compared for their miR profile. (b) Overview of miR profiling of EBNA2-transfected U2932 cells. (c) A comparison of principally altered miRs in U2932 EBVGFP cl-B and U2932 EBNA2 cl-2. The differences for both miR-21 and miR-146a expression in comparison to the parental U2932 cells were statistically significant (P<0.05), as measured by Student’s t-test. The number of independent samples is n=3. (d) List of miRs statistically differentially expressed in U2932 EBVGFP cl-B (EBNA2 expressor) vs U2932 EBVGFP cl-A (EBNA2 non expressor) upper panel and in EBNA2 overexpressed U2932 EBNA2 cl-2 vs parental vector U2932 MPA vector (lower panel). The P-value is ⩽0.05 for the miRNA listed (Mann–Whitney test).
