Figure 3
From: Differential regulation of miR-21 and miR-146a by Epstein–Barr virus-encoded EBNA2
pAKT expression and the effect of miR-21 knockdown in EBNA2 expressing U2932 cells. (a) pAKT and total AKT expression was analyzed by immunoblotting in U2932 EBV-convertant and EBNA2-transfected clone. The role of miR-21 in the regulation of pAKT was further studied by knocking down miR-21 by transfecting antisense LNA miR-21 oligonucleotide. RPMI 8226 and LCLAKE were used as negative and positive controls, respectively. (b) Expression of miR-21 as analyzed by taqman qPCR in the corresponding cells as in (a). The figure represents mean of three experiments, each performed in triplicates. The values are normalized to an internal U6 RNA control.
