Figure 5
From: Differential regulation of miR-21 and miR-146a by Epstein–Barr virus-encoded EBNA2
The effect of LMP1 on miR-146a expression in U2932- and EBNA2-transfected cells. (a) U2932 and U2932 EBNA2 cl-2 cells were transiently cotransfected with pSG5 LMP1 and pEGFP-N1 expression vectors. The GFP-positive and -negative cells were sorted. The P2 quadrant (green) and P3 quadrant (blue) show GFP-positive and -negative cells, respectively. (b) EBNA2, LMP1 and lamin B expression as seen by immunoblotting in GFP-sorted parental U2932 and its EBNA2-transfected derivative U2932 EBNA2 cl-2. (c) Expression of miR-146a by Taqman qPCR in corresponding cell lines. The figure represents a mean of three experiments.
