Figure 6
From: Differential regulation of miR-21 and miR-146a by Epstein–Barr virus-encoded EBNA2
IRAK1 and type I interferon expression in EBV-infected and EBNA2/LMP1-transfected cells: (a) Immunoblotting was performed to evaluate IRAK1 protein expression. A monoclonal anti-IRAK1 antibody is used. EBNA2 and LMP1 expression was verified by using respective monoclonal antibody. (b) qPCR was employed to assess IRAK1 transcript levels in EBV-converted and EBNA2/LMP1-transfected U2932 cell, using IRAK1-specific taqman primers. The experiment represents mean of three experiments, each performed in triplicates, from two different RNA extraction. The values are normalized against an internal GAPDH mRNA control. (c) IFNA4 (top panel) and IFNA2 (lower panel) were quantified by qRT-PCR in total RNA-derived from U2932 EBV-infected and -transfected with the empty vector (MPA), with EBNA2 or with LMP1. Levels of IFNA2 and IFNA4 mRNAs were normalized to the GAPDH level using the formula 2−ΔΔCt; the values shown are means±s.d. of triplicate determinations.
