Table 1 Xenograft samples after first transplantation and serial transplantations

From: Successful xenografts of AML3 samples in immunodeficient NOD/shi-SCID IL2Rγ−/− mice

Patient no.

1st transplantation

 

No. of cells/mouse ( × 10 6 ) mouse priming

Time of evaluation (week)

%hCD45 + a

%CD33 + a Gate on hCD45

%CD19 + a Gate on hCD45

%CD34 + a Gate on hCD45

PML-RAR (Bcr)

No. of mice with AML/no. of mice injected

1

0.5

6/10/12

0.5/15/46

100/100/99

0/0/0

ND/ND/14

Bcr3

1/2

 

Irradiation

 

—/ND/NDb

—/ND/NDb

—/ND/NDb

—/ND/NDb

  

2

5

6/20/24

0/63/80

0/100/100

0/0/0

ND/26/30

Bcr3

6/6

 

Irradiation

 

0/74/72

0/100/100

0/0/0

ND/43/45

  
   

0/60/85

0/100/100

0/0/0

ND/39/24

  
   

0/44/58

0/100/100

0/0/0

ND/27/35

  
   

0/29/58

0/100/100

0/0/0

ND/12/14

  
   

0.5/—b

100/—b

0/—b

ND/—b

  

3

5

6/16/20

0/0.24/5.7

0/100/100

0/0/0

ND/ND/9

LowRNA

1/5

 

Bisulfex

 

0±0/0±0/0±0c

0±0/0±0/0±0c

0±0/0±0/0±0c

ND/ND/ND

  

4d

10

6/12/16

0/1.5/1.8

0/100/100

0/0/0

ND/ND/ND

LowRNA

5/5

 

Irradiation

 

0/0.09/0.08

0/100/100

0/0/0

ND/ND/ND

  
   

0/0.05/0.05

0/100/100

0/0/0

ND/ND/ND

  
   

0/0.05/0.39

0/100/100

0/0/0

ND/ND/ND

  
   

0/0.03/0.03

0/100/100

0/0/0

ND/ND/ND

  

5

10

6/12/16

0±0/0±0/0±0c

0±0/0±0/0±0c

0±0/0±0/0±0c

ND/ND/ND

LowRNA

0/4

 

Bisulfex

       

6d

10

6/16/20

0/2±0.7/5±1.8c

0/34±3.7/17±3.3c

0/66±3.7/83±3.3c

ND/ND/11±1.8c

LowRNA

0/10

 

Irradiation

       

7d

5

6/16/20

0/0.4±3.7/1.5±0.11c

0/2±4/33±2c

0/98±4/67±2c

ND/ND/ND

LowRNA

0/6

 

Bisulfex

       

8

10

6/16/20

0/0.5±0/2±0.75c

0/22±3/15±3c

0/78±3/85±3c

ND/ND/ND

LowRNA

0/9

 

Bisulfex

       

Patient no.

Serial transplantations

 

Serial transplantation

No. of cellse injected/mouse( × 106)

No. of mice

Time of evaluation (week)

%CD34 + Gate on hCD45 (last week)

%CD33 + Gate on hCD45 (last week)

PML-RAR (Bcr) (last week)

No. of mice with AML/no. of mice injected

1

First transplant

0.5

2

6/10/2012

14±0f

99±0f

BCR3

1/2

 

Second transplant

1

6

6

20±0.7

100±0

LowRNA

6/6

 

Third transplant

0.04

12

6/9

28±2.2

100±0

BCR3

12/12

 

Fourth transplant

1

2

6

34±6

100±0

LowRNA

2/2

2

First transplant

5

6

16

30±5

100±0

BCR3

6/6

 

Second transplant

1

9

16

22±4

100±0

BCR3

7/9

  1. Abbreviations: AML, acute myeloid leukemia; BM, bone marrow; ND, not done because of low RNA yield in mouse BM samples; PB, peripheral blood.
  2. aImmunophenotype of PB samples at first transplantation taken at the different weeks of evaluation as described in column 3.
  3. bMouse died before complete evaluation.
  4. cMean±s.e.m. of all mice evaluated.
  5. dSimilar data were obtained from the PB and BM patient samples.
  6. ehCD45+ cell equivalent.
  7. Data of the last week of evaluation.
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