Figure 3
From: Antileukemic activity of nuclear export inhibitors that spare normal hematopoietic cells
KPT-185 promotes arrest at G1 and S stages of the cell cycle and induces apoptotic cell death. (a) Relative cell viability of AML cell lines (Cell Titer Glo assay) upon treatment with KPT-185 for 3 days. Mean±s.d. values. (b) Annexin V and PI staining of the MV4-11 cells upon treatment with 30, 60 and 120 nM KPT-185 for 24 h. (c) Mean±s.d. percentages of cells in sub-G1, G0/G1, S, and G2/M stages of the cell cycle. The results of three biologic replicates are shown. (d) TUNEL vs PI (DNA content) histograms of MV4-11 cells, treated either with DMSO or 60 nM KPT-185 for 24 h. White boxes indicate the TUNEL-positive cells in G1, S and G2/M stages of cell cycle. (e) Mean±s.d. percentages of TUNEL-positive MV4-11 cells in each cell cycle stage upon treatment with 60 nM KPT-185 are shown. The results represent three biologic replicates.
