Table 4 The EuroClonality uniform system for technical description

From: EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations

Type of profile per tube (in duplicate)

Technical description per tube

Optional: more detailed technical description a

No peaks/bands (but: poor DNA quality)

No (specific) product, poor DNA quality

 

No peaks/bands (w/o background)

No (specific) product

Nonspecific product(s) (… nt)

One or two reproducible clonal peaks/bandsb

Clonal (… nt)

Weak clonal (… nt)

Clonal (… nt)+polyclonal background (Gaussian curve/smear)

One or two non-reproducible (clear) peaks/bandsb

Pseudoclonal

 

Multiple (n⩾3) non-reproducible peaks/bandsb

Pseudoclonal

 

Multiple (n⩾3) reproducible peaks/bandsb

Multiple products (n= …) (… nt)

 

Gaussian curve/smearc (with or without minor reproducible peaks/bandsb)

Polyclonal (not clonalc)

Irregular polyclonal (not clonalc)

Pattern that cannot be categorized as one of the above

Not evaluabled

 
  1. Abbreviations: HD, heteroduplex; nt, nucleotide.
  2. aExamples of more detailed technical description options that can be chosen by the user.
  3. bIn HD analysis the number of bands does not necessarily reflect the number of different PCR products, as additional HDs can be formed between products.
  4. cIn HD analysis a polyclonal smear may not always be smooth or clear, despite specific product in gel; hence this is scored as ‘not clonal’.
  5. dIn <5% of PCR results the description per tube cannot be made.
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