Table 5 The EuroClonality uniform system for molecular conclusion

Overall technical description for all Ig or TCR targets	Molecular interpretation/conclusion	Optional: more detailed molecular interpretation ^a
No (specific) product, poor DNA quality	Not evaluable, due to poor DNA quality
No (specific) product	No rearrangement in Ig/TCR targets detected
Clonal (… nt)^f	Clonality detected	Clonality detected (biallelic products) Clonality detected (biclonality) Clonality detected (minor clonal product) Clonality detected (isolated rearrangement) clonality detected (with caution, plus advice for follow-up analysis/new sample) Clonality detected in addition to background of B/T cells
Pseudoclonal (one or more non-reproducible products)	No clonality detected, suggestive of low template amount
Multiple reproducible products (n⩾3)^b	Oligoclonality/multiple clones detected	Dominant clone in oligo/polyclonal background
Polyclonal (not clonal^c)	Polyclonality detected (no clonality detected^c)	Polyclonality detected plus minor clone of unknown significance^d
Not evaluable	Not evaluable^e

Abbreviations: HD, heteroduplex; Ig, immunoglobulin; IGK, IG kappa; nt, nucleotide; TCR, T-cell receptor; TCRB, TCR beta.
^aExamples of more detailed molecular interpretation options that can be chosen by the user.
^bFor IGK and TCRB loci up to four clonal products may be compatible with one clone.
^cIn HD analysis a polyclonal smear may not always be clearly detectable, despite specific product in agarose gel; hence this is scored as ‘not clonal’.
^dFor those cases in which minor reproducible peaks/bands are detected in the polyclonal background.
^eIn <5% of cases the molecular interpretation cannot be made.
^fClonal peaks/bands are not necessarily seen for every Ig/TCR target analyzed to reach the molecular conclusion ‘clonality detected’.

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