Figure 1 | Leukemia

Figure 1

From: Preclinical activity of LBH589 alone or in combination with chemotherapy in a xenogeneic mouse model of human acute lymphoblastic leukemia

Figure 1

In vitro efficacy of LBH589 in ALL cell lines. (a) Apoptosis induced by LBH589 in TOM-1 and MOLT-4 cell lines measure by the activation of caspase-3 by fluorescence-activated cell sorting (FACS). (b) Survival of TOM-1 and MOLT-4 cells after treatment with LBH589 at doses of 50 nM for 4 days. (c) Acetylation of H3 and H4 and phosphorylation of H2AX in TOM-1 and MOLT-4 cells after treatment with increasing concentrations of LBH589 measure by western blot analysis. (d) Time-course analysis of activation of caspase-3 in TOM-1 and MOLT-4 ALL cells after treatment with 50 nM of LBH589. (e) Acetylation of H3 and H4 and phosphorylation of H2AX in TOM-1 and MOLT-4 cells after treatment with LBH589 measure by western blot analysis. The mean±s.d. of at least three independent experiments are shown in (a, b, d) while a representative experiment is depicted in (c, e). Total H3 was used as loading control in (c, e). *Significant statistical differences between groups.

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