Figure 5
CD20 expression level determined the susceptibility of primary CLL cells to CDC. (a) The MFI of CD20 was determined by flow cytometric analysis in all patient samples. The MFI of CD20 varied highly among the 37 patient samples, namely from 3412 to 38 143. (b) CD20 expression levels were significantly higher (P<0.0001) in CDC responder patients (gray bar) than in CDC non-responder patients (black bar). The statistical analysis was performed by t-test for unpaired data. Bars: s.e.m. (c) Plotting the MFI of CD20 against the lysis induced by 20 μg/ml RTX showed a highly significant correlation (P<0.0001). (d) Similarly, when the MFI of CD20 was plotted against the lysis induced by 20 μg/ml RTX combined with hSCR18–20, a significant correlation was obtained (P<0.0001).
