Figure 1 | Leukemia

Figure 1

From: Establishing optimal quantitative-polymerase chain reaction assays for routine diagnosis and tracking of minimal residual disease in JAK2-V617F-associated myeloproliferative neoplasms: a joint European LeukemiaNet/MPN&MPNr-EuroNet (COST action BM0902) study

Figure 1

Summary of QC rounds used to identify JAK2-V617F assays with the best performance profile. Seven successive QC rounds, designated QC1–7, were conducted involving the assessment of plasmid standards and DNA derived from dilutions of cell lines harboring JAK2-V617F (HEL or UKE-1) in cells with wild-type JAK2 (K562 or PB mononuclear cells), abbreviated: HEL/K562, UKE-1/PBMNC, as indicated. Assays found to exhibit consistently poorer performance in multiple test laboratories were not taken forward to subsequent QC rounds—reasons for assay exclusion are provided. Details of the JAK2-V617F assays (designated Assays 1–9) and control gene assays (Albumin, Cyclophilin) are provided in Supplementary Table 1.

Back to article page