Figure 2 | Leukemia

Figure 2

From: Establishing optimal quantitative-polymerase chain reaction assays for routine diagnosis and tracking of minimal residual disease in JAK2-V617F-associated myeloproliferative neoplasms: a joint European LeukemiaNet/MPN&MPNr-EuroNet (COST action BM0902) study

Figure 2

Plasmid standards. In QC rounds QC3–7, in addition to the assessment on cell line dilutions, JAK2 and control gene assays were tested on serial dilutions of plasmid standards. The efficiencies of JAK2-V617F Assays 2, 5 and 7 were found to be superior to that of Assay 6, with slope values much closer to −3.32 (the slope associated with a PCR reaction with maximal efficiency). y-axis: Mean cycle threshold (Ct) values; x-axis: log dilution.

Back to article page