Table 1 p53 status of CLL patients with 17p deletion

From: Antibody-dependent cellular cytotoxicity of the optimized anti-CD20 monoclonal antibody ublituximab on chronic lymphocytic leukemia cells with the 17p deletion

Patient no.

del17p (FISH) a

p53 functional assessment b

TP53 mutationc

1

52%

Unfunctional

c.673-2A>T/p.IVS7-2A>T (intron 6)

2

97%

Unfunctional

c.417G>T/p.V173L (exon 5)

3

90%

Unfunctional

c.524G>A/p.R175H (exon 5)

4

56%

Unfunctional

c.584T>C, p.I195T (exon 6) ; c.673-2A>C, p.IVS7-2A>C (intron 6) ∼20%; c.842A>T, p.D281V (exon 8)

5

86%

Unfunctional

c.614A>C/p.Y205S (exon 6)

6

73%

Unfunctional

c.716 A>G, p.N239S (exon 7)

7

81%

Unfunctional

c.794T>C, p.L265P (exon 8)

8

87%

Unfunctional

Unmutated (exons 4–10)

9

89%

Unfunctional

Unmutated (exons 4–10)

10

79%

Unfunctional

c.743G>A/p.R248E (exon 7)

11

93%

Unfunctional

c.921G>T/p.G244V (exon 7)

12

83%

Unfunctional

c.Del 754-762CTCACCATC/p.Del252-254LTI (exon 7); c.818G>A/p.R273H (exon 8)

13

14%

Unfunctional

Unmutated (exons 4–10)

14

23%

Unfunctional

c.536A>G/p.H179R (exon 5)

  1. Abbreviations: del, deletion; FISH, fluorescent in situ hybridization: % of interphasic cells with abnormality.
  2. aDel(17)(p13.1) or del17p was detected using Vysis LSI p53 FISH Probe from Abbott Molecular.
  3. bThe assessment of the functional status of p53 in CLL cells was based on induction of p21 and p53 protein expression using etoposide and nutlin-3a (Le Garff-Tavernier7 in the manuscript).
  4. cDetection of TP53 mutation was performed by Sanger sequencing.
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