Table 1 Summary of mutations and SNVs in CRBN and DDB1 in MM cell lines and patients
IMiD-sensitive MM cell lines | CRBN nucleotide position and change | CRBN amino-acid position and change | DDB1 nucleotide position and change | DDB1 amino-acid position and change |
|---|---|---|---|---|
H929 parental, U266, EJM, SKMM2 | None | None | ||
OPM-2 | G1209A silent heterozygous | T403T | None | |
KMS-12-BM parental | T735C silent homozygous | Y245Y | None | |
Intrinsic IMiD-resistant MM cell lines | ||||
LP1 | None | None | ||
RPMI 8226 | None | None | ||
JJN3 | None | None | ||
Paired sensitive and acquired resistant cell lines | ||||
H929 sensitive control | None | None | ||
H929 4 resistant clones | None | None | ||
KMS-12-BM sensitive control | T735C silent homozygous | Y245Y | None | |
KMS-12-BM LEN-resistant | T735C silent homozygous | Y245Y | None | |
KMS-12-BM POM-resistant | T735C silent homozygous | Y245Y | None | |
MM1S sensitive | None | None | ||
MM1S/R10R LEN-resistant | None | None | ||
ANBL-6 sensitive | None | A909T heterozygous | E303D | |
None | C153T silent heterozygous | P51P (rs2230356) | ||
ANBL-6 LEN-resistant | G745T heterozygous | D249Y | A909T heterozygous | E303D |
None | C153T silent heterozygous | P51P (rs2230356) | ||
Patient samples (90) | ||||
Newly diagnosed (24) | T735C silent homozygous, C219T silent heterozygous | 2 × Y245Y (rs17027638), 1 × H73H | C153T silent heterozygous, C939T silent heterozygous | 2 × P51P (rs2230356), 1 × C313C (rs150106100) |
RRMM (30) | T735C silent homozygous | 2 × Y245Y (rs17027638) | C153T silent heterozygous | 4 × P51P (rs2230356) |
LEN-resistant RRMM (36) | None | None | ||
