Figure 1
From: APOBEC3 signature mutations in chronic lymphocytic leukemia
Clustered mutations are present in IgV-Mut CLL samples. (a) Analysis of whole genome sequencing data by rainfall plots shows intermutational distances (IMDs) in four individual CLL cases with unmutated (CLL1 and 2) and mutated IgV (CLL3 and 4). Mutations are shown as dots with the y axis giving the distance to the next downstream mutation on the same chromosome. The genomic position of the respective mutation is given on the x axis, with chromosomes (1–22, x,y) spaced by vertical lines. Total mutations and mean IMDs are indicated to the right of each rainfall plot. Clustered mutations (defined as ⩾3 mutations spaced by ⩽10kb) are indicated with arrows. Clustered mutations inside the Ig loci are marked with black arrows and outside Ig loci with white arrows. Local sequence context of (b) unclustered and (c) clustered C>T transitions from CLL data from (a). (b) The probability of occurrence of individual bases upstream and downstream of a C mutated to T is shown for all mutated Cs (upper panel), for Cs outside CpGs (middle panel) and outside CpG islands (lower panel) as defined by the UCSC Table Browser tool. (c) Sequence context of all clustered C>T mutations (total) are shown and for clustered C>T mutations at Ig (middle panel) and non-Ig loci (lower panel).
