Figure 2
From: APOBEC3 signature mutations in chronic lymphocytic leukemia
APOBEC3 family members are expressed in CLL. (a) SYBR green qRT-PCR data from cDNA of 10 IgV-Mut, 8 IgV-UM CLL samples and 5 healthy controls (HD) using APOBEC3A-H (abbreviated: A3A-H)-specific primer sets normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) levels (scatter dot blot with median values indicated as bars). Statistically significant values are indicated within the graph. (b) Analysis of APOBEC3A, APOBEC3B and APOBEC3H expression levels in peripheral blood mononucleated cells from primary IgV-Mut (IDs 4865, 6846, 4902, 3185, 4940) and IgV-UM (IDs 4481, 5247) patients were determined by immunoblotting after 8 days under cell culture conditions with (+CpG) or without (untreated) CpG treatment. Arrows to the left indicate specific bands. Tubulin was used as a loading control.
