Table 1 Certified copy number concentrations of double-stranded plasmid DNA and their uncertainties for the six ERM-AD623 dilutions

From: A certified plasmid reference material for the standardisation of BCR–ABL1 mRNA quantification by real-time quantitative PCR

CRM

Copy number a concentration of the plasmid (cp/μl)

uchar,rel (%)

ubb,rel (%)

ults,rel (%)

uCRM,rel (%)

uCRM (cp/μl)

ERM-AD623a

1.08 × 106

3.28

3.57

2.75

11.15

0.13 × 106

ERM-AD623b

1.08 × 105

3.58

2.88

2.21

10.19

0.11 × 105

ERM-AD623c

1.03 × 104

3.01

2.59

2.81

9.73

0.10 × 104

ERM-AD623d

1.02 × 103

2.66

2.47

2.11

8.40

0.09 × 103

ERM-AD623e

1.04 × 102

3.06

2.75

2.43

9.56

0.10 × 102

ERM-AD623f

10.0

4.28

4.37

3.83

14.42

1.5

  1. Abbreviations: CRM, certified reference material; ubb,rel, relative uncertainity related to potential between-unit heterogeneity of the material; uchar,rel, relative uncertainity related to the charecterisation study; uCRM, expanded uncertainity of the certified value (with k=2); uCRM,rel, relative expanded uncertainity of the certified value (with k=2); ults,rel, relative uncertainity related to potential degradation during long-term storage (24 months at −20 °C).
  2. aAs the copy number concentrations refer to the copy numbers of double-stranded plasmid, these numbers should be doubled when calibrating quantitative real-time PCR experiments that measure single-stranded cDNA samples.
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