Figure 4
Impairment of IRF4 signaling by miR-125b-5p. (a) Western blot analysis of BLIMP-1 in SK-MM-1 cells transfected with miR-125b-5p or miR-NC. Analysis was performed 24 and 48 h after cell transfection. γ-Tubulin was used as protein loading control. (b) Western blot analysis of BLIMP-1 in lysates from SK-MM-1 cells co-transfected with either BLIMP-1 ORF expression vector or IRF4 ORF expression vector or an empty vector and miR-125b-5p or miR-NC (48-h time point). (c) CCK-8 assay of SK-MM-1 cells co-transfected with either BLIMP-1 ORF expression vector or an empty vector and miR-125b-5p or miR-NC (72-h time point). (d) Western blot analysis of c-Myc, Casp-10 and cFLIP in SK-MM-1 cells transfected with miR-125b-5p or miR-NC. Analysis was performed 24 and 48 h after cell transfection. GAPDH or γ-Tubulin were used as protein loading controls. (e) Western blot analysis of c-Myc, Casp-10 and cFLIP in lysates from SK-MM-1 cells co-transfected with either IRF4 ORF expression vector or an empty vector and miR-125b-5p or miR-NC (48-h time point). GAPDH was used as protein loading control. All the experiments were performed in triplicate. Representative pictures are shown. (f) Explanatory cartoon of miR-125b-5p-mediated impairment of IRF4 signaling.
