Table 1 Summary of reference gene numbers required for scoring deep molecular response

From: Laboratory recommendations for scoring deep molecular responses following treatment for chronic myeloid leukemia

 

MR 4

MR 4.5

MR 5

Minimum sum of reference gene transcripts irrespective of whether BCR-ABL1 is detected or nota

10 000 ABL1 24 000 GUSB

32 000 ABL1 77 000 GUSB

100 000 ABL1 240 000 GUSB

BCR-ABLIS level for positive samplesb

⩽0.01%

⩽0.0032%

⩽0.001%

  1. aNumbers of reference gene transcripts in same volume of cDNA that is tested for BCR-ABL1. The minimum number in any individual replicate should be 10 000 ABL1 or 24 000 GUSB.
  2. bProvided that the minimum reference gene copy numbers in the row above are fulfilled.
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