Figure 4 | Leukemia

Figure 4

From: A complementary role of multiparameter flow cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study

Figure 4

ClonoSEQ high-throughput sequencing (HTS) shows good linearity to one CLL cell in one million leukocytes. Analysis of three CLL cases diluted into leukocytes from leucodepletion filters in serial 1:10. Each CLL clone was tagged with two sequences, one productive and one non-productive. The plot shows the CLL sequence as a percentage of nucleated genomes. Each case has a different marker shape (square for case 1, circle for case 2 and diamond for case 3) with no fill for the productive sequence data, gray fill for the non-productive sequence and black fill for the average. For log-transformed data above the limit of detection, linearity=1.12, correlation coefficient Pearson R=0.98.

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