Table 1 Target values for markers used in CLL MRD analysis

From: A complementary role of multiparameter flow cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study

Antigen

Typical expression in CLL a

Control populations in normal peripheral blood

Minimum b relative fluorescence intensity (preferred)

  

Positive

Negative

 

CD5

Positive (>20%)

CD3+ T cells

CD19+ B cells

>30 (>65)

CD20

Weak

CD19+ B cells

CD3+ T-cells

>10 (>20)

CD43

Positive (>20%)

CD3+ T cells

CD20+ B cells

>15 (>40)

CD79b

Weak

CD20+ B cells

CD3+ T-cells

>15 (>30)

CD81

Weak

CD3+ T cells

Granulocytes

>12 (>20)

  1. aFor the typical expression pattern, 'positive' indicates percentage of cells positive compared with control, and 'weak' indicates at least 20% reduction in fluorescence intensity relative to the median expression observed with a reference population of polyclonal B cells using the same antibody.
  2. bThe minimum relative fluorescence intensity would provide separation of CLL cells from normal B cells in >95% of cases (see Figure 3) with a preferred relative fluorescence intensity being the level at which >99% of cases have optimal separation of CLL cells from normal B cells.
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