Figure 2 | Leukemia

Figure 2

From: Proteasome inhibitor-adapted myeloma cells are largely independent from proteasome activity and show complex proteomic changes, in particular in redox and energy metabolism

Figure 2

Protein and RNA expression for UPR signaling pathway. AMO-1 and its derivatives adapted to bortezomib (AMO-BTZ) and carfilzomib (AMO-CFZ) have been cultured for 2 weeks without proteasome inhibitors before RNA and protein was collected. (a) Level of spliced (s), unspliced (u) and total (t) XBP1 in the cell lines. (b) Presence of activated XBP1 depicted as a ratio of spliced XBP1 vs unspliced XBP1 in the cell lines. (c) Representative western blotting bands for protein levels of UPR signaling proteins in the cell lines. For panels a and b, data are presented as mean±s.d. of three independent replicates. Statistical significance <0.05 is marked by asterisks.

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