Table 2 Proteomic comparison of AMO-1 cells with AMO-BTZ and AMO-CFZ

From: Proteasome inhibitor-adapted myeloma cells are largely independent from proteasome activity and show complex proteomic changes, in particular in redox and energy metabolism

Change

GO term

P-value range

Number of proteins

Mean fold change (range)

AMO-BTZ

 Upregulated

Protein catabolism

10−8

19

1.7 (2.3–1.4)

 

Redox homeostasis

10−7–10−6

25

1.9 (6.1–1.4)

 

Folding and complex formation

10−3–10−2

53

2.0 (19.7–1.4)

 

Transport/signaling

10−3–10−2

116

1.7 (4.3–1.4)

 

Metabolic regulation

10−3–10−2

114

1.8 (9.2–1.4)

 Downregulated

Transcription/translation

10−2

63

0.6 (0.7–0.2)

 

Differentiation

10−2

13

0.6 (0.7–0.4)

 

Cytoskeleton organization

10−2

22

0.5 (0.7–0.1)

 

Metabolic regulation

10−2

45

0.6 (0.7–0.4)

 

Transport/signaling

10−2

89

0.5 (0.7–0.1)

 

Apoptosis

10−1

8

0.5 (0.7–0.3)

AMO-CFZ

 Upregulated

Protein catabolism

10−8

22

1.7 (2.7–1.4)

 

Transport/signaling

10−6–10−2

85

1.9 (12.1–1.4)

 

Metabolic regulation

10−6–10−2

110

1.8 (4.6–1.4)

 

Folding and complex formation

10−3–10−2

39

2.2 (13.9–1.4)

 

Redox homeostasis

10−3–10−2

15

2.2 (4.3–1.4)

 Downregulated

Apoptosis

10−3

7

0.5 (0.7–0.2)

 

Metabolic regulation

10−3–10−2

58

0.5 (0.7–0.2)

 

Transport/signaling

10−2

72

0.5 (0.7–0.1)

 

Transcription/translation

10−2

58

0.6 (0.7–0.2)

 

Differentiation

10−2

10

0.5 (0.7–0.2)

 

Cytoskeleton organization

10−2

24

0.5 (0.7–0.1)

HL60–BTZ

 Upregulated

Protein catabolism

10−15

23

1.7 (2.7–1.4)

 

Transport/signaling

10−10–10−2

80

1.8 (6.8–1.4)

 

Metabolic regulation

10−3–10−2

65

1.8 (4.5–1.4)

 

Folding and complex formation

10−2

28

1.8 (3.9–1.4)

 

Redox homeostasis

10−2

20

1.9 (4.4–1.4)

 Downregulated

Transport/signaling

10−5

40

0.6 (0.7–0.3)

 

Metabolic regulation

10−3–10−2

34

0.6 (0.7–0.3)

 

Transcription/translation

10−2

64

0.6 (0.7–0.2)

 

Redox homeostasis

10−2

16

0.6 (0.7–0.2)

 

Cytoskeleton organization

10−2

12

0.6 (0.7–0.4)

  1. Abbreviation: GO, gene ontology. Supervised clustering of GO terms into functionally related clusters of biological processes found for the upregulated and downregulated proteins in the cell lines adapted to bortezomib (AMO-BTZ, HL60-BTZ) and carfilzomib (AMO-CFZ). P-values range is obtained from the unsupervised clusters of different GO terms (see Supplementary Table S1) clustered in each category.
Back to article page