Figure 4
The influence of CALR mutation on hematopoietic stem/progenitor cells. (a) The proportion of HSCs and progenitors. Compared with WT mice (n=9), CALRdel52-TG mice (n=10) show increased frequencies of long-term (LT)-HSC, short-term (ST)-HSC, LSK, common myeloid progenitor (CMP), erythromegakaryocyte progenitors (MEP) and megakaryocyte progenitor (MKP). *P<0.05 and **P<0.01 vs WT mice. (b) Enumeration of colonies and serial replating capacity of 5 × 104 BM cells from WT (n=9) or TG (n=10) mice. Compared with the BM cells from WT mice, those from TG mice generated larger numbers of colonies in vitro. TG BM cells did not have enhanced sequential colony-replating capacity. **P<0.01 vs WT mice. (c) Serial transplantation assays. WT or CALRdel52 BM cells (B6-CD45.2) together with competitor WT BM cells (B6-CD45.1) were transplanted in a 1:1 ratio into lethally irradiated recipients (B6-CD45.1) and then recipient 1 × 106 BM cells were transplanted into a second set of lethally irradiated recipients (B6-CD45.1). The chimerism of donor-derived CD45.2+ cells in peripheral blood after the first and the second transplantations at the indicated time points is shown. **P<0.01 vs WT cells. All data are presented as means±s.e.m. The two-tailed Student’s t-test was used (a and b). For the comparison of the chimerism, analysis of variance with repeated measures was used (c).
