Figure 4

Cytarabine-resistant SHI-1 cells exhibited enhanced sensitivity to glucocorticoids and upregulation of NR3C1. (a) SHI-1 parental and cytarabine-resistant variants showing sensitivity to three glucocorticoids—dexamethasone, methylprednisolone and prednisolone. (b) Correlation was calculated between DSS of cytarabine and individual glucocorticoids in SHI-1 cytarabine-resistant variants; r indicates Pearson correlation with P<0.05. (c, d, e) A targeted small-scale drug testing experiment was performed in SHI-1 parental and cytarabine-resistant variants to validate the high-throughput DSRT data on glucocorticoid sensitivity. Glucocorticoids were tested with nine doses in triplicates with same parameters as in the DSRT assay. The bars represent mean ±s.d. (f) TAS was calculated and correlated with gene expression profiles in SHI-1 parental and cytarabine-resistant variants; r indicates Spearman correlation, where P=0.02. (g) NR3C1 protein expression was analyzed using western blotting in SHI-1 parental and cytarabine-resistant cells. The integrated intensities of protein bands were quantified by Odyssey software and normalized against GAPDH.